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Molecular and Cellular Biology, April 2005, p. 3247-3260, Vol. 25, No. 8
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.8.3247-3260.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

The p53 Tumor Suppressor Protein Represses Human snRNA Gene Transcription by RNA Polymerases II and III Independently of Sequence-Specific DNA Binding

Anastasia A. Gridasova and R. William Henry*

Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, Michigan

Received 7 September 2004/ Returned for modification 28 October 2004/ Accepted 20 January 2005

Human U1 and U6 snRNA genes are transcribed by RNA polymerases II and III, respectively. While the p53 tumor suppressor protein is a general repressor of RNA polymerase III transcription, whether p53 regulates snRNA gene transcription by RNA polymerase II is uncertain. The data presented herein indicate that p53 is an effective repressor of snRNA gene transcription by both polymerases. Both U1 and U6 transcription in vitro is repressed by recombinant p53, and endogenous p53 occupancy at these promoters is stimulated by UV light. In response to UV light, U1 and U6 transcription is strongly repressed. Human U1 genes, but not U6 genes, contain a high-affinity p53 response element located within the core promoter region. Nonetheless, this element is not required for p53 repression and mutant p53 molecules that do not bind DNA can maintain repression, suggesting a reliance on protein interactions for p53 promoter recruitment. Recruitment may be mediated by the general transcription factors TATA-box binding protein and snRNA-activating protein complex, which interact well with p53 and function for both RNA polymerase II and III transcription.

* Corresponding author. Mailing address: Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, MI 48824. Phone: (517) 353-3980. Fax: (517) 353-9334. E-mail: henryrw{at}msu.edu.

Molecular and Cellular Biology, April 2005, p. 3247-3260, Vol. 25, No. 8
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.8.3247-3260.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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  • Gu, L., Husain-Ponnampalam, R., Hoffmann-Benning, S., Henry, R. W. (2007). The Protein Kinase CK2 Phosphorylates SNAP190 to Negatively Regulate SNAPC DNA Binding and Human U6 Transcription by RNA Polymerase III. J. Biol. Chem. 282: 27887-27896 [Abstract] [Full Text]  
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