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Molecular and Cellular Biology, January 2005, p. 206-219, Vol. 25, No. 1
0270-7306/05/$08.00+0     doi:10.1128/MCB.25.1.206-219.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.

Core Promoter Binding by Histone-Like TAF Complexes

Hanshuang Shao ,1,{dagger},{ddagger} Merav Revach,1,{dagger} Sandra Moshonov,1 Yael Tzuman,1 Kfir Gazit,1 Shira Albeck,2 Tamar Unger,2 and Rivka Dikstein1*

Department of Biological Chemistry,1 Israel Structural Proteomic Center, The Weizmann Institute of Science, Rehovot, Israel2

Received 14 June 2004/ Returned for modification 13 September 2004/ Accepted 30 September 2004

A major function of TFIID is core promoter recognition. TFIID consists of TATA-binding protein (TBP) and 14 TBP-associated factors (TAFs). Most of them contain a histone fold domain (HFD) that lacks the DNA-contacting residues of histones. Whether and how TAF HFDs contribute to core promoter DNA binding are yet unresolved. Here we examined the DNA binding activity of TAF9, TAF6, TAF4b, and TAF12, which are related to histones H3, H4, H2A, and H2B, respectively. Each of these TAFs has intrinsic DNA binding activity adjacent to or within the HFD. The DNA binding domains were mapped to evolutionarily conserved and essential regions. Remarkably, HFD-mediated interaction enhanced the DNA binding activity of each of the TAF6-TAF9 and TAF4b-TAF12 pairs and of a histone-like octamer complex composed of the four TAFs. Furthermore, HFD-mediated interaction stimulated sequence-specific binding by TAF6 and TAF9. These results suggest that TAF HFDs merge with other conserved domains for efficient and specific core promoter binding.

* Corresponding author. Mailing address: Department of Biological Chemistry, The Weizmann Institute of Science, Rehovot 76100, Israel. Phone: 972-8-9342117. Fax: 972-8-9344118. E-mail: rivka.dikstein{at}weizmann.ac.il.

{dagger} H.S. and M.R. contributed equally to this study.

{ddagger} Present address: Department of Pathology, University of Pittsburgh, Pittsburgh, Pa.

Molecular and Cellular Biology, January 2005, p. 206-219, Vol. 25, No. 1
0022-538X/05/$08.00+0     doi:10.1128/MCB.25.1.206-219.2005
Copyright © 2005, American Society for Microbiology. All Rights Reserved.



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