This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wu, S. Y.
Right arrow Articles by McLeod, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wu, S. Y.
Right arrow Articles by McLeod, M.

 Previous Article  |  Next Article 

Mol. Cell. Biol., Mar 1995, 1479-1488, Vol 15, No. 3
Copyright © 1995, American Society for Microbiology

The sak1+ gene of Schizosaccharomyces pombe encodes an RFX family DNA- binding protein that positively regulates cyclic AMP-dependent protein kinase-mediated exit from the mitotic cell cycle

SY Wu and M McLeod
State University of New York Health Science Center at Brooklyn, 11203.

In Schizosaccharomyces pombe, meiosis is initiated by conditions of nutrient deprivation. Mutations in genes encoding elements of the cyclic AMP-dependent protein kinase (cAPK) pathway interfere with meiosis. Loss-of-function alleles of genes that stimulate the activity of cAPK allow cells to bypass the normal requirement of starvation for conjugation and meiosis. Alternatively, loss-of-function alleles of genes that inhibit cAPK lead to the inability to undergo sexual differentiation. The cgs1+ gene encodes the regulatory subunit of cAPK, and the cgs2+ gene encodes a cyclic AMP phosphodiesterase. Thus, both genes encode proteins which negatively regulate the activity of cAPK. Loss of either cgs1 or cgs2 prevents haploid cells from conjugating and diploid cells from undergoing meiosis. In addition to these defects, cells are unable to enter stationary phase. We describe a novel gene, sak1+, which when present on a plasmid overcomes the aberrant phenotypes associated with unregulated cAPK activity. Genetic analysis of sak1+ (suppressor of A-kinase) reveals that it functions downstream of cyclic AMP-dependent protein kinase to allow cells to exist the mitotic cycle and enter either stationary phase or the pathway leading to sexual differentiation. The sak1+ gene is essential for cell viability, and a null allele causes multiple defects in cell morphology and nuclear division. Thus, sak1+ is an important regulatory element in the life cycle of S. pombe. Sequence analysis shows that the predicted product of the sak1+ gene is an 87-kDa protein which shares homology to the RFX family of DNA-binding proteins identified in humans and mice. One member of this family, RFX1, is a transcription factor for a variety of viral and cellular genes.


This article has been cited by other articles:

  • Senti, G., Swoboda, P. (2008). Distinct Isoforms of the RFX Transcription Factor DAF-19 Regulate Ciliogenesis and Maintenance of Synaptic Activity. Mol. Biol. Cell 19: 5517-5528 [Abstract] [Full Text]  
  • Seto, H., Hayashi, Y., Kwon, E., Taguchi, O., Yamaguchi, M. (2006). Antagonistic regulation of the Drosophila PCNA gene promoter by DREF and Cut.. GENES CELLS 11: 499-512 [Abstract] [Full Text]  
  • Lubelsky, Y., Reuven, N., Shaul, Y. (2005). Autorepression of Rfx1 Gene Expression: Functional Conservation from Yeast to Humans in Response to DNA Replication Arrest. Mol. Cell. Biol. 25: 10665-10673 [Abstract] [Full Text]  
  • Efimenko, E., Bubb, K., Mak, H. Y., Holzman, T., Leroux, M. R., Ruvkun, G., Thomas, J. H., Swoboda, P. (2005). Analysis of xbx genes in C. elegans. Development 132: 1923-1934 [Abstract] [Full Text]  
  • Davidson, M. K., Shandilya, H. K., Hirota, K., Ohta, K., Wahls, W. P. (2004). Atf1-Pcr1-M26 Complex Links Stress-activated MAPK and cAMP-dependent Protein Kinase Pathways via Chromatin Remodeling of cgs2+. J. Biol. Chem. 279: 50857-50863 [Abstract] [Full Text]  
  • Yuasa, T., Hayashi, T., Ikai, N., Katayama, T., Aoki, K., Obara, T., Toyoda, Y., Maruyama, T., Kitagawa, D., Takahashi, K., Nagao, K., Nakaseko, Y., Yanagida, M. (2004). An interactive gene network for securin-separase, condensin, cohesin, Dis1/Mtc1 and histones constructed by mass transformation. GENES CELLS 9: 1069-1082 [Abstract] [Full Text]  
  • Otsuki, K., Hayashi, Y., Kato, M., Yoshida, H., Yamaguchi, M. (2004). Characterization of dRFX2, a novel RFX family protein in Drosophila. Nucleic Acids Res 32: 5636-5648 [Abstract] [Full Text]  
  • Stiefel, J., Wang, L., Kelly, D. A., Janoo, R. T. K., Seitz, J., Whitehall, S. K., Hoffman, C. S. (2004). Suppressors of an Adenylate Cyclase Deletion in the Fission Yeast Schizosaccharomyces pombe. Eukaryot Cell 3: 610-619 [Abstract] [Full Text]  
  • Jeong, H. T., Ozoe, F., Tanaka, K., Nakagawa, T., Matsuda, H., Kawamukai, M. (2004). A Novel Gene, msa1, Inhibits Sexual Differentiation in Schizosaccharomyces pombe. Genetics 167: 77-91 [Abstract] [Full Text]  
  • Araki, R., Takahashi, H., Fukumura, R., Sun, F., Umeda, N., Sujino, M., Inouye, S.-I. T., Saito, T., Abe, M. (2004). Restricted Expression and Photic Induction of a Novel Mouse Regulatory Factor X4 Transcript in the Suprachiasmatic Nucleus. J. Biol. Chem. 279: 10237-10242 [Abstract] [Full Text]  
  • Schmitt, E. K., Bunse, A., Janus, D., Hoff, B., Friedlin, E., Kurnsteiner, H., Kuck, U. (2004). Winged Helix Transcription Factor CPCR1 Is Involved in Regulation of {beta}-Lactam Biosynthesis in the Fungus Acremonium chrysogenum. Eukaryot Cell 3: 121-134 [Abstract] [Full Text]  
  • Nakayama, A., Murakami, H., Maeyama, N., Yamashiro, N., Sakakibara, A., Mori, N., Takahashi, M. (2003). Role for RFX Transcription Factors in Non-neuronal Cell-specific Inactivation of the Microtubule-associated Protein MAP1A Promoter. J. Biol. Chem. 278: 233-240 [Abstract] [Full Text]  
  • Dubruille, R., Laurencon, A., Vandaele, C., Shishido, E., Coulon-Bublex, M., Swoboda, P., Couble, P., Kernan, M., Durand, B. (2002). Drosophila Regulatory factor X is necessary for ciliated sensory neuron differentiation. Development 129: 5487-5498 [Abstract] [Full Text]  
  • Morotomi-Yano, K., Yano, K.-i., Saito, H., Sun, Z., Iwama, A., Miki, Y. (2002). Human Regulatory Factor X 4 (RFX4) Is a Testis-specific Dimeric DNA-binding Protein That Cooperates with Other Human RFX Members. J. Biol. Chem. 277: 836-842 [Abstract] [Full Text]  
  • McLeod, M., Shor, B., Caporaso, A., Wang, W., Chen, H., Hu, L. (2000). Cpc2, a Fission Yeast Homologue of Mammalian RACK1 Protein, Interacts with Ran1 (Pat1) Kinase To Regulate Cell Cycle Progression and Meiotic Development. Mol. Cell. Biol. 20: 4016-4027 [Abstract] [Full Text]  
  • Schmitt, E. K., Kuck, U. (2000). The Fungal CPCR1 Protein, Which Binds Specifically to beta -Lactam Biosynthesis Genes, Is Related to Human Regulatory Factor X Transcription Factors. J. Biol. Chem. 275: 9348-9357 [Abstract] [Full Text]  
  • Sengupta, P. K., Ehrlich, M., Smith, B. D. (1999). A Methylation-responsive MDBP/RFX Site Is in the First Exon of the Collagen alpha 2(I) Promoter. J. Biol. Chem. 274: 36649-36655 [Abstract] [Full Text]  
  • Katan-Khaykovich, Y., Shaul, Y. (1998). RFX1, a Single DNA-binding Protein with a Split Dimerization Domain, Generates Alternative Complexes. J. Biol. Chem. 273: 24504-24512 [Abstract] [Full Text]  
  • Dimitrov, K., Sazer, S. (1998). The Role of fnx1, a Fission Yeast Multidrug Resistance Protein, in the Transition of Cells to a Quiescent G0 State. Mol. Cell. Biol. 18: 5239-5246 [Abstract] [Full Text]  
  • Stettler, S, Warbrick, E, Prochnik, S, Mackie, S, Fantes, P (1996). The wis1 signal transduction pathway is required for expression of cAMP-repressed genes in fission yeast. J. Cell Sci. 109: 1927-1935 [Abstract]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»